EXTRACTION AND CHARACTERIZATION OF NATURAL DYES FROM ANTHOCLEISTA VOGELLI, JUSTICIA CARNEA AND MANGIFERA INDICA

Abstract

The extraction of natural dyes from Anthocleista vogelii, Justicia carnea and Mangifera indica are reported. The optimization of the extraction process was evaluated using acetone, ethanol, and water, pressurized liquid extraction and Soxhlet extraction. The optimal natural dye extraction was observed with acetone using pressurized liquid extraction for 1 h. The extracted natural dyes were isolated using column chromatography and were characterized by HPLC. The Anthocleista vogelii HPLC test for alkaloids revealed the presence of Hordenine, Cytisine and Galanthamine; Anthocleista vogelii HPLC test for flavoids revealed the presence of Benzoic acid, Isoquercetin, Rutin and Chlorogenic acid; Anthocleista vogelii HPLC test for phenol revealed no compound. Justicia carnea HPLC test for alkaloids revealed the presence of Hordenine, Cytisine, Methyl Jasmonate and Galanthamine; Justicia carnea HPLC test for flavonoids revealed the presence of Benzoic acid, Isoquercetin, Rutin, Hesperidin, Chlorogenic acid and Ellagic acid;
Justicia carnea HPLC test for phenols revealed the presence of Ferulic acid. Mangifera indica HPLC test for flavonoids revealed the presence of Benzoic acid, Isoquercetin, Rutin and Chlorogenic acid; Mangifera indica HPLC test for phenol revealed the presence of Eugenol and Ferulic acid. The toxicity test LD50 for the natural dyes were evaluated. It was observed that the LD50 value of the Anthocleista vogelii was above 5000 mg/kg and that no deaths was recorded; the LD50 value of the Justicia carnea was above 5000 mg/kg and that no deaths was recorded; and the LD50 value of the Mangifera indica was 5054.77 mg/kg and two deaths was recorded