STAINING POTENTIALS OF LOCALLY SOURCED CURCUMA LONGA, CNESTIS FERRUGINEA AND GARCINIA KOLA ON LIVER AND KIDNEY TISSUES

Authors

  • N. J. C Okolie
  • A. A Ngokere
  • I. B Enweani
  • O Okechi

Keywords:

Curcuma longa, Cnestis ferruginea, Garcinia cola, ethanolic extract, aqueous extract

Abstract

The use of eosin in histopathology may be restricted due to the discovery of its hazardous nature. Moreover, most developing countries can no longer afford the ever-increasing cost of the stains. Consequently, the need for evaluation of staining potentials of Curcuma longa rhizome, Cnestis ferruginea stem barks and Garcinia kola mesocap becomes paramount. In the present study, sohext extracts of the plant products were substituted for eosin (as counter stains) in the modified haematoxylin and eosin methods for the staining of kidney and liver tissue slides in order to study their staining potentials. Results showed that 100 mg/ml freshly prepared C. longa rhizome extracts was most effective in staining the slides; giving excellent cytoplasm staining. C. ferruginea extracts produced faintly stained pale red colouration of the cytoplasm. Tissue sections stained with G. kola pods extracts showed fairly good cyto-architectural distinctions. When each of the tissue slides was stained with only a single stain (mono staining) and 6-month old plant extracts, there was reduction in the intensities and clarity of features. The suitability of the plant products as counter stains as observed in the study could be attributable to their acidic nature which naturally stained the basic cytoplasm contents.

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Published

2022-06-23

How to Cite

Okolie , N. J. C., Ngokere , A. A., Enweani , I. B., & Okechi , O. (2022). STAINING POTENTIALS OF LOCALLY SOURCED CURCUMA LONGA, CNESTIS FERRUGINEA AND GARCINIA KOLA ON LIVER AND KIDNEY TISSUES. NIGERIAN JOURNAL OF SCIENCE AND ENVIRONMENT, 18(1). Retrieved from https://delsunjse.com/index.php/njse/article/view/68